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Retinoic acid modulates peripheral blood helper innate lymphoid cell composition in vitro in patients with multiple sclerosis


. 2024 Nov 17:398:578489.


doi: 10.1016/j.jneuroim.2024.578489.


Online ahead of print.

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Altuğ Özkoşar et al.


J Neuroimmunol.


.

Abstract

This study investigates the frequency and numbers of circulating helper innate lymphoid cells (ILCs) in untreated relapsing-remitting multiple sclerosis (RRMS) patients, focusing on intracellular IL-10 and CCR6 expressions under IL-2, IL-33, and retinoic acid (RA) stimulation in vitro and their associations with clinical features in RRMS. In RRMS patients, ILC1 levels were notably higher upon IL-2 + IL-33 + RA stimulation, while ILC2 levels, particularly the c-Kit+ ILC2 and CCR6+ ILC2 subsets, were significantly lower compared to unstimulated conditions. Additionally, IL-10+ ILC1 levels were elevated. The ratios of IL-10+ ILC1/ILC1, c-Kit+ ILC2/c-Kit ILC2, and CCR6+ ILC2/ILC2 were associated with the progression index (PI) in RRMS patients.


Keywords:

Innate lymphoid cell; Innate lymphoid cell plasticity; Interleukin-10; Multiple sclerosis; Retinoic acid.

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Conflict of interest statement

Declaration of competing interest All authors state that they do not have any conflicting financial or non-financial interest related to the study.


Fonte original PubMed

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